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SRX18273182: Spacer in vitro integration into pCRISPR7 with 1/2 saturating concentration Alba
1 ILLUMINA (Illumina MiSeq) run: 84,344 spots, 8.4M bases, 3.2Mb downloads

Design: Amplicon library with one degenerate primer and one spacer-specific primer
Submitted by: UConn Health
Study: Pyrococcus furiosus COM1 Raw sequence reads
PRJNA901420 • SRP408047 • All experiments • All runs
show Abstracthide Abstract
Pyrococcus furiosus MNase protected DNA was sequenced to determine patterns of protein binding to the chromosome, particularly near CRISPR arrays. Histones were identified as a key binding protein and were used in an in vitro integration study to determine their influence on CRISPR spacer integration. Both the in vitro integration sequencing data and the MNase-Seq data are available here.
Sample: Histone profile for Pyrococcus furious
SAMN31712712 • SRS15833182 • All experiments • All runs
Organism: Pyrococcus furiosus
Library:
Name: pCRISPR7_invitro_Alba_half
Instrument: Illumina MiSeq
Strategy: AMPLICON
Source: SYNTHETIC
Selection: PCR
Layout: SINGLE
Runs: 1 run, 84,344 spots, 8.4M bases, 3.2Mb
Run# of Spots# of BasesSizePublished
SRR2229930884,3448.4M3.2Mb2023-03-25

ID:
25277340

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